News & Events
New Cas3 enzyme for CRISPR technology created
- April 12, 2019
- Posted by: Wiley M. Wagner
- Category: Science
The technology of sequential gene modification called CRISPR has been used for quite a long time, and therefore it becomes clear that its subsequent modification is only a matter of time. Some time ago, several new enzymes were introduced to work with the system, which can even more accurately and quickly pull out, copy and delete individual genes and genetic markers from the genome compared to the traditional Cas9 enzyme, which can only make small “cuts” and only once However, today another new enzyme for the CRISPR system was introduced, which can literally cut through large pieces of the tested DNA.
This new enzyme is called Cas3 and is significantly different from both Cas9 and Cas12a and Cas12b. It is necessary first to talk about the last two, which were created using a non-pathogenic bacterial base, and therefore have greater safety for the DNA itself and its carrier. However, they are deprived of the opportunity to work with a large amount of information at a time, which was the reason for the development of a conceptually new molecule and a new model of functioning of the CRISPR technology.
The new Cas3 enzyme functions as a peculiar shredder of genetic markers and genes, possessing the ability to capture and work with more than 100,000 genes and genetic combinations, which in itself cannot but surprise, considering the similar non-pathogenic environment of origin of this molecule. Preliminary laboratory tests demonstrated that the Cas3 enzyme copes well with processing a large amount of genetic combinations, both in a human embryonic cell and in HAP1 cells.
And this, in turn, means the possibility of significantly faster information processing and refinement of some additional features and functions of modern technology for CRISPR genetic modification – scientists assume that soon they will begin their studies in rodents, in order to make sure that the new Cas3 genetic enzyme will be truly excellent and multifunctional in all possible conditions of its work.